mouse monoclonal antibodies against survivin  (Novus Biologicals)

Journal: Cells

Article Title: An Insight into Survivin in Relevance to Hematological, Biochemical and Genetic Characteristics in Tobacco Chewers with Oral Squamous Cell Carcinoma

doi: 10.3390/cells12101444

Figure Lengend Snippet: Photomicrographs of immunohistochemical staining for survivin in oral squamous cell carcinoma tissues ( A – D ) and normal buccal mucosa ( E , F ). Immunohistochemical staining shows positive staining in brown. Photographs captured with a digital camera at 100× and 400× magnification under identical conditions.

Article Snippet: Antigen retrieval was performed with citrate buffer pH 9 using a decloaker, primary mouse monoclonal antibodies against survivin (IHC 668, GenomeMe, GenomeMe Lab Inc., Richmond, BC, Canada), and a secondary antibody linked to horseradish peroxidase, which specifically binds to the primary antibody.

Techniques: Immunohistochemical staining, Staining

Journal: BioMed Research International

Article Title: Improvement of Inflammation through Antioxidant Pathway of Gardeniae Fructus 50% EtOH Extract (GE) from Acute Reflux Esophagitis Rats

doi: 10.1155/2020/4826176

Figure Lengend Snippet: Western blot analysis of (a) survivin, (b) cytochrome c, (c) caspase-3, (d) Bax, and (e) Bcl-2 expressions. Nor: normal rats, Con: reflux esophagitis control rats, GL: GE 50 mg/kg treated reflux esophagitis rats, and GH: GE 100 mg/kg treated reflux esophagitis rats. (f) All data are expressed means ± SEM, ( n = 6) rats per group. Significance: ∗ p < 0.05, ∗∗ p < 0.01 versus RE control rat values.

Article Snippet: Mouse monoclonal antibodies against survivin (1 : 1,000, NB 500-205) were purchased from Novus Biologicals (Littleton, CO, USA).

Techniques: Western Blot, Reflux, Control

Journal: Iranian Biomedical Journal

Article Title: Downregulation of Kinesin Spindle Protein Inhibits Proliferation, Induces Apoptosis and Increases Chemosensitivity in Hepatocellular Carcinoma Cells

doi: 10.6091/ibj.1386.2014

Figure Lengend Snippet: Sequences of the primers for RT-PCR and real-time qRT-PCR

Article Snippet: The membranes were blocked in blocking solution BSA (Sigma-Aldrich, St. Louis, MO, USA) and incubated with mouse monoclonal antibody against Eg5 or KSP (1:200), mouse monoclonal antibody against cyclin D1 (1:500), mouse monoclonal antibody against Bcl-2 (1:500), and mouse monoclonal antibody against Survivin (1:500) (all were bought from Abcam, Cambridge, ENG, UK) at room temperature for 1 h. After washing, the membranes were incubated with horseradish peroxidase-linked goat anti-mouse IgG (1:2000, Abcam, Cambridge, UK) for 45 min.

Techniques:

Journal: Iranian Biomedical Journal

Article Title: Downregulation of Kinesin Spindle Protein Inhibits Proliferation, Induces Apoptosis and Increases Chemosensitivity in Hepatocellular Carcinoma Cells

doi: 10.6091/ibj.1386.2014

Figure Lengend Snippet: The mRNA expression of Cyclin D1, Bcl-2 and Survivin in Hep3B cells. (a) The mRNA expressions of Cyclin D1, Bcl-2 and Survivin in Hep3B cells were detected by RT-PCR after 72 h of siRNA transfection. Electrophoretic profile of PCR products of the Cyclin D1 (358 bp) Bcl-2 (174 bp), Survivin (145 bp) and β-actin (680 bp) genes. β-actin was used as a housekeeping gene control. (b) The mRNA levels of Cyclin D1, Bcl-2 and Survivin in Hep3B cells were also determined by real-time qRT-PCR. The mRNA expression of these genes was normalized with β-actin. Each bar represents the mean value ± standard deviation (SD) of triplicate. * P <0.05 compared to untreated cell group.

Article Snippet: The membranes were blocked in blocking solution BSA (Sigma-Aldrich, St. Louis, MO, USA) and incubated with mouse monoclonal antibody against Eg5 or KSP (1:200), mouse monoclonal antibody against cyclin D1 (1:500), mouse monoclonal antibody against Bcl-2 (1:500), and mouse monoclonal antibody against Survivin (1:500) (all were bought from Abcam, Cambridge, ENG, UK) at room temperature for 1 h. After washing, the membranes were incubated with horseradish peroxidase-linked goat anti-mouse IgG (1:2000, Abcam, Cambridge, UK) for 45 min.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection, Control, Quantitative RT-PCR, Standard Deviation

Journal: Iranian Biomedical Journal

Article Title: Downregulation of Kinesin Spindle Protein Inhibits Proliferation, Induces Apoptosis and Increases Chemosensitivity in Hepatocellular Carcinoma Cells

doi: 10.6091/ibj.1386.2014

Figure Lengend Snippet: The protein expression of Cyclin D1, Bcl-2 and Survivin in Hep3B cells. (a) The protein expressions of Cyclin D1, Bcl-2 and Survivin in Hep3B cells were measured by Western blot analyses after transfection. β-actin was used as a housekeeping gene control. The size of each protein was indicated. (b) Densitometric analysis of these three proteins was made relative to β-actin. Each bar represents the mean value ± standard deviation (SD) of triplicate. * P <0.05 compared to control cell group

Article Snippet: The membranes were blocked in blocking solution BSA (Sigma-Aldrich, St. Louis, MO, USA) and incubated with mouse monoclonal antibody against Eg5 or KSP (1:200), mouse monoclonal antibody against cyclin D1 (1:500), mouse monoclonal antibody against Bcl-2 (1:500), and mouse monoclonal antibody against Survivin (1:500) (all were bought from Abcam, Cambridge, ENG, UK) at room temperature for 1 h. After washing, the membranes were incubated with horseradish peroxidase-linked goat anti-mouse IgG (1:2000, Abcam, Cambridge, UK) for 45 min.

Techniques: Expressing, Western Blot, Transfection, Control, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: PCR primers and conditions.

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques:

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: Survivin mRNA expression in thyroid tissues. (a) Weak or negative expression of survivin mRNA in benign goiter tissues; (b) strong survivin mRNA expression in carcinoma tissues. K + , positive control, human colorectal carcinoma cell line ‘Caco’.

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques: Expressing, Positive Control

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: Relationship of survivin expression and various prognostic factors in 75 patients with thyroid carcinoma.

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques: Expressing

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: Correlation between the transcript expression of endogenous survivin and various clinicopathological parameters. Thyroid cancer samples expressed higher transcript expression of endogenous survivin when compared to the benign goiters; the expression was significantly correlated with the grade of tumor invasiveness (pT), local lymph node metastasis (N) and distant metastasis (M) of the thyroid carcinoma tissues.

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques: Expressing

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: Immunostaining patterns of survivin in frozen human thyroid tissue samples. (a) Thyroid goiter tissue with a loss of expression of survivin. (b) Strong immunostaining in a primary PTC (pT4N0M0). (c) A primary FTC (pT4N1M0) showing moderate survivin protein expression. (d) Uniformly intense staining of survivin protein expression in UTC (pT4N1M0) tissue. PTC, papillary thyroid cancer; FTC, follicular thyroid cancer; UTC, undifferentiated thyroid cancer.

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques: Immunostaining, Expressing, Staining

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: Correlation between the protein expression of endogenous survivin and various clinicopathological parameters. Protein expression of endogenous survivin was significantly correlated with the grade of tumor invasiveness T and local lymph node metastasis (N); and with distant metastasis (M) of thyroid carcinoma tissues but this value did not achieve statistical significance.

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques: Expressing

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: Effect of survivin siRNA on FTC-133 cells as detected using immunocytochemistry. (a) Untreated cells; (b) cells treated with mismatch siRNA; (c) cells treated with sis-100 siRNA; (d) cells treated with sis-248 siRNA

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques: Immunocytochemistry

Journal: International Journal of Molecular Medicine

Article Title: Targeting of the anti-apoptotic gene survivin in human thyroid carcinoma

doi: 10.3892/ijmm.2012.1046

Figure Lengend Snippet: Western blot analysis indicates reduced survivin expression following siRNA treatment in FTC-133 cells.

Article Snippet: Subsequently, replicate sections were incubated at 4°C overnight with the specific mouse monoclonal antibody against human survivin (clone D8; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at a dilution of 1:200.

Techniques: Western Blot, Expressing